Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-PKC antibody [EPR16898] - BSA and Azide free
See all PKC primary antibodies
Description

Rabbit monoclonal [EPR16898] to PKC - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human, Recombinant fragment
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
General notes
ab250108 is the carrier-free version of ab179522.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16898
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/2000 dilution

Lane 1 : Active human PKC alpha full length protein
Lane 2 : Active human PKC beta 1 full length protein
Lane 3 : Active human PKC beta 2 full length protein
Lane 4 : Active human PKC gamma full length protein
Lane 5 : Active human PKC delta full length protein
Lane 6 : Active human PKC eta full length protein
Lane 7 : Active human PKC epsilon full length protein
Lane 8 : Active human PKC theta full length protein
Lane 9 : Active human PKC mu full length protein

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Predicted band size: 77 kDa

Exposure time: 2 seconds

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag; Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag; Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag; Active human PKC gamma full length protein (ab60842) contains aa1-677 with GST-tag; Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag; Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag; Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag; Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag; Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
Immunocytochemistry - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

This data was developed using ab179522, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling PKC alpha + beta + gamma with ab179522 at 1/200 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image shows cytoplasmic staining on K562 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).The negative controls are as follows:-
-ve control 1: - ab179522 at 1/200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/2000 dilution

Lane 1 : Active human PKC zeta full length protein
Lane 2 : Active human PKC iota full length protein

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 77 kDa

Exposure time: 3 minutes

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Active human PKC zeta full length protein (ab60848) contains aa1-592 with GST-tag; Active human PKC iota full length protein (ab60850) contains aa1-596 with GST-tag.
Other - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

This data was developed using ab179522, the same antibody clone in a different buffer formulation.
Recombinant PKC isoforms were tested for reactivity by western blot, and are marked as positive (+) or negative (-).
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg
Lane 3 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg
Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution

Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

All lanes : Anti-PKC antibody [EPR16898] (ab179522) at 1/5000 dilution

Lane 1 : L6 (Rat skeletal muscle cell line) whole cell lysate
Lane 2 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 3 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

Anti-PKC antibody [EPR16898] (ab179522) at 1/1000 dilution + Rat brain whole tissue lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

This data was developed using ab179522, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

This data was developed using ab179522, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed NIH/3T3 (Mouse embyro fibroblast cells) cells labeling PKC alpha + beta + gamma with ab179522 at 1/360 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)

This data was developed using ab179522, the same antibody clone in a different buffer formulation.PKC alpha + beta + gamma was immunoprecipitated from 1mg of Rat brain extract with ab179522 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab179522 at 1/1000 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody was used as secondary antibody at 1/1000 dilution.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-PKC antibody [EPR16898] - BSA and Azide free (ab250108)