Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)
Key features and details
- Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody
See all PKA alpha + beta (catalytic subunits) primary antibodies -
Description
Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197) -
Host species
Rabbit -
Specificity
ab59218 detects endogenous levels of PKA alpha + beta (catalytic subunit) only when phosphorylated at threonine 197. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic phosphopeptide derived from human PKA alpha + beta (catalytic subunit)around the phosphorylation site of threonine 197 (T-W-TP-L-C).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab59218 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
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Western blot - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)All lanes : Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218) at 1/500 dilution
Lane 1 : mouse brain cell extract
Lane 2 : mouse brain cell extract with immunising peptide
Predicted band size: 41 kDa
Observed band size: 41 kDa
