Antibodies, Proteins, Kits and Reagents for Life Science

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)

Key features and details

Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
Suitable for: IHC-P, WB
Reacts with: Mouse, Human
Isotype: IgG

Product name

Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody
See all PKA alpha + beta (catalytic subunits) primary antibodies
Description

Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
Host species

Rabbit
Specificity

ab59218 detects endogenous levels of PKA alpha + beta (catalytic subunit) only when phosphorylated at threonine 197.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Mouse

See all applications and species data
Immunogen

Synthetic phosphopeptide derived from human PKA alpha + beta (catalytic subunit)around the phosphorylation site of threonine 197 (T-W-T^P-L-C).

Application	Species
IHC-P	Human
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Without Mg+2 and Ca+2
Concentration information loading...
Purity

Immunogen affinity purified
Purification notes

ab59218 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality

Polyclonal
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)

ab59218 at 1/50 dilution staining PKA alpha + beta (catalytic subunit) in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence (left) or presence (right) of the immunising peptide.
Western blot - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218)

All lanes : Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab59218) at 1/500 dilution

Lane 1 : mouse brain cell extract
Lane 2 : mouse brain cell extract with immunising peptide

Predicted band size: 41 kDa
Observed band size: 41 kDa

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