Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free
See all Pit1 primary antibodies
Description

Rabbit monoclonal [EPR23555-203] to Pit1 - BSA and Azide free
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Rat
ICC/IF	Rat
IHC-Fr	Mouse
IHC-P	Mouse
IP	Mouse
WB	Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: GH3 whole cell lysate, Mouse pituitary tissue lysate. IHC-P: Mouse pituitary tissue. IHC-Fr: Mouse pituitary tissue. ICC/IF: GH3 cells. Flow Cyt: GH3 cells. IP: Mouse pituitary tissue lysate. GH3 whole cell lysate.
General notes
ab273057 is the carrier-free version of ab273048. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm.

Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23555-203
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Pit1 with ab273048 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse pituitary (PMID:21123951). Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pituitary tissue labeling Pit1 with ab273048 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). Nuclear staining on mouse pituitary is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Western blot - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

All lanes : Anti-Pit1 antibody [EPR23555-203] (ab273048) at 1/1000 dilution

Lane 1 : Mouse pituitary tissue lysate
Lane 2 : Mouse liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 33 kDa
Observed band size: 33 kDa

Exposure time: 81 seconds

The expression profile observed is consistent with what has been described in the literature (PMID: 21123951).
Negative control: Mouse liver (PMID: 21123951).

Blocking/Diluting buffer and concentration 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Pit1 was immunoprecipitated from 0.35 mg mouse pituitary tissue lysate 10µg with ab273048 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273048 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse pituitary tissue lysate 10µg.

Lane 2: ab273048 IP in mouse pituitary tissue lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273048 in mouse pituitary tissue lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 23 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized GH3 (Rat pituitary epithelial cell line) cells labelling Pit1 with ab273048 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing nuclear staining in GH3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 2µg/ml dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Flow Cytometry - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized GH3 (Rat pituitary epithelial cell line) cells labelling Pit1 with ab273048 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Pit1 was immunoprecipitated from 0.35 mg GH3 (Rat pituitary epithelial), whole cell lysate 10µg with ab273048 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273048 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: GH3 (Rat pituitary epithelial), whole cell lysate 10µg.

Lane 2: ab273048 IP in GH3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273048 in GH3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Pit1 with ab273048 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution (Green). No staining on mouse liver is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 (2µg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Pit1 with ab273048 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with Hematoxylin.

Negative control: No staining in mouse cerebrum (PMID:21123951).

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).
Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)