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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC

Anti-PICOT antibody (ab226396)

Anti-PICOT antibody (ab226396)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PICOT
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PICOT antibody
    See all PICOT primary antibodies
  • Description

    Rabbit polyclonal to PICOT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human PICOT aa 285-335. The exact sequence is proprietary.
    Database link: O76003

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH-3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
  • General notes

    Previously labelled as TXNL2. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226396 was affinity purified using an epitope specific to PICOT immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway

Images

  • Western blot - Anti-PICOT antibody (ab226396)
    Western blot - Anti-PICOT antibody (ab226396)
    All lanes : Anti-PICOT antibody (ab226396) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • Immunoprecipitation - Anti-PICOT antibody (ab226396)
    Immunoprecipitation - Anti-PICOT antibody (ab226396)

    PICOT was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226396 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226396 at 0.4 µg/ml.

    Lane 1: ab226396 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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