Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864)
Key features and details
- Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Phosphoribosyl pyrophosphate amidotransferase antibody
See all Phosphoribosyl pyrophosphate amidotransferase primary antibodies -
Description
Rabbit polyclonal to Phosphoribosyl pyrophosphate amidotransferase -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 283-467 of Human Phosphoribosyl pyrophosphate amidotransferase (NP_002694).
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Positive control
- WB: HepG2 and HCT116 whole cell lysates. IF: HeLa whole cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864)All lanes : Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Phosphoribosyl pyrophosphate amidotransferase antibody (ab125864)Immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate labeling Phosphoribosyl pyrophosphate amidotransferase with ab125864 at 1/500 dilution. Costained with Hoechst 33342 (Bottom panel).