Anti-Peroxiredoxin 2/PRP antibody (ab59539)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 2/PRP
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Peroxiredoxin 2/PRP antibody
See all Peroxiredoxin 2/PRP primary antibodies -
Description
Rabbit polyclonal to Peroxiredoxin 2/PRP -
Host species
Rabbit -
Specificity
ab59539 reacts with Peroxiredoxin 2/PRP.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Cynomolgus monkey, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Peroxiredoxin 2/PRP aa 20-31.
Sequence:VVDGAFKEVKLS
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Positive control
- WB: HeLa, whole tissue lysate prepared from murine liver. ICC: HeLa cells. IHC-P: Human colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Peroxiredoxin 2/PRP antibody (ab59539)Anti-Peroxiredoxin 2/PRP antibody (ab59539) at 1/500 dilution + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 37 kDa, 49 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-Peroxiredoxin 2/PRP antibody (ab59539)ICC/IF image of ab59539 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39539, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody (ab59539)ab59539 (1/2000) staining peroxiredoxin 2/PRP in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of submucosal infiltrating leukocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Western blot - Anti-Peroxiredoxin 2/PRP antibody (ab59539) Image courtesy of an anonymous Abreview.All lanes : Anti-Peroxiredoxin 2/PRP antibody (ab59539) at 1/5000 dilution
All lanes : Whole tissue lysate prepared from murine liver
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Predicted band size: 22 kDa
Observed band size: 22,45 kDa why is the actual band size different from the predicted?
