Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDLIM1/CLP36 antibody [EPR7186(B)]
    See all PDLIM1/CLP36 primary antibodies

  • Description

    Rabbit monoclonal [EPR7186(B)] to PDLIM1/CLP36

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PDLIM1/CLP36 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human heart, L6, 293T, Caco 2, Saos 2, C2C12, Rat lung and Mouse lung lysates; Saos 2 cells. ICC/IF: 293T cells

  • General notes

     

     

     This product was previously labelled as PDLIM1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    Western blot - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    All lanes : Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015) at 1/1000 dilution

    Lane 1 : Human heart cell lysate
    Lane 2 : L6 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Caco 2 cell lysate
    Lane 5 : Saos 2 cell lysate
    Lane 6 : C2C12 cell lysate
    Lane 7 : Rat lung cell lysate
    Lane 8 : Mouse lung cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 36 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    Immunocytochemistry/ Immunofluorescence - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    Immunocytochemistry/ Immunofluorescence analysis of 293T (human embryonic kidney epithelial cell) cells labeling PDLIM1/CLP36 with purified ab129015 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

  • Flow Cytometry - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    Flow Cytometry - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

    ab129015, at 1/100 dilution, staining PDLIM1/CLP36 in permeabilized Saos 2 cells by Flow Cytometry or a Rabbit IgG negative control (green).

  • OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    OI-RD Scanning - Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)
    Anti-PDLIM1/CLP36 antibody [EPR7186(B)] (ab129015)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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