Anti-PDCD7 antibody [EPR8231] (ab131258)
![Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145596-anti-pdcd7-antibody-epr8231-immunofluorescence.jpg "Anti-PDCD7 antibody [EPR8231] (ab131258)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8231] to PDCD7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PDCD7 antibody [EPR8231]
See all PDCD7 primary antibodies -
Description
Rabbit monoclonal [EPR8231] to PDCD7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human PDCD7 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, Jurkat, MCF7 and Caco 2 cell lysates; MCF7 cells; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.10 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8231 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunofluorescent analysis of MCF7 cells labelling PDCD7 with ab131258 at /150 dilution.
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![Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145598-anti-pdcd7-antibody-epr8231-western-blot.jpg)
Western blot - Anti-PDCD7 antibody [EPR8231] (ab131258)All lanes : Anti-PDCD7 antibody [EPR8231] (ab131258) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145597-anti-pdcd7-antibody-epr8231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling PDCD7 with ab131258 antibody at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145595-anti-pdcd7-antibody-epr8231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunohistochemical analysis using ab131258 showing positive staining in Normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145594-anti-pdcd7-antibody-epr8231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunohistochemical analysis using ab131258 showing positive staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145593-anti-pdcd7-antibody-epr8231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunohistochemical analysis using ab131258 showing positive staining in Normal breast tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145592-anti-pdcd7-antibody-epr8231-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD7 antibody [EPR8231] (ab131258)Immunohistochemical analysis using ab131258 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-145591-anti-pdcd7-antibody-epr8231.jpg)
OI-RD Scanning - Anti-PDCD7 antibody [EPR8231] (ab131258) -
![Anti-PDCD7 antibody [EPR8231] (ab131258)](https://www.abcam.com/ps/products/131/ab131258/Images/ab131258-9-benefits-of-recombinant-antibodies.png)
Anti-PDCD7 antibody [EPR8231] (ab131258)
