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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PDCD4 (phospho S457) antibody (ab226849)

Anti-PDCD4 (phospho S457) antibody (ab226849)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to PDCD4 (phospho S457)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat
  • Isotype: IgG

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Overview

  • Product name

    Anti-PDCD4 (phospho S457) antibody
    See all PDCD4 primary antibodies
  • Description

    Rabbit polyclonal to PDCD4 (phospho S457)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PDCD4 (C terminal) (phospho S457). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q53EL6

  • Positive control

    • WB: NIH/3T3 treated with PDGF, PC-12, Rat2 whole cell lysate. IHC-P: Mouse spleen and rat intestine tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Images

  • Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)
    Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)
    All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/500 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 51 kDa



    10% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution. 
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

  • Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)
    Western blot - Anti-PDCD4 (phospho S457) antibody (ab226849)
    All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) treated with 5 ng/ml PDGF for 24 hours whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 51 kDa



    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 (phospho S457) antibody (ab226849)

    Immunohistochemical analysis of paraffin-embedded rat intestine tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution. 
    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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