Anti-PDCD4 (phospho S457) antibody (ab226849)
Key features and details
- Rabbit polyclonal to PDCD4 (phospho S457)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-PDCD4 (phospho S457) antibody
See all PDCD4 primary antibodies -
Description
Rabbit polyclonal to PDCD4 (phospho S457) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PDCD4 (C terminal) (phospho S457). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q53EL6 -
Positive control
- WB: NIH/3T3 treated with PDGF, PC-12, Rat2 whole cell lysate. IHC-P: Mouse spleen and rat intestine tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 51 kDa10% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min. -
All lanes : Anti-PDCD4 (phospho S457) antibody (ab226849) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) treated with 5 ng/ml PDGF for 24 hours whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 51 kDa10% SDS-PAGE gel.
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Immunohistochemical analysis of paraffin-embedded rat intestine tissue staining PDCD4 (phospho Ser457) protein at nucleus with ab226849 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.