Anti-PCGF1 antibody (ab84108)
Key features and details
- Rabbit polyclonal to PCGF1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCGF1 antibody
See all PCGF1 primary antibodies -
Description
Rabbit polyclonal to PCGF1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PCGF1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94828) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: MCF7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowZebrafishApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Relevance
PCGF1 is a component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. It represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). PCGF1 als promotes cell cycle progression and also enhances cell proliferation, thus it may have a positive role in tumor cell growth. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 539040 Cow
- Entrez Gene: 84759 Human
- Entrez Gene: 69837 Mouse
- Entrez Gene: 312480 Rat
- Omim: 610231 Human
- SwissProt: Q2YDF9 Cow
- SwissProt: Q9BSM1 Human
- SwissProt: Q8R023 Mouse
see all -
Alternative names
- PCGF1 antibody
- 2010002K04Rik antibody
- Lethal(3)73AH, Drosophila, homolog of antibody
see all
Images
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Western blot - Anti-PCGF1 antibody (ab84108)Anti-PCGF1 antibody (ab84108) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 105 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (1)
ab84108 has been referenced in 1 publication.
- Yang L et al. MicroRNA-320a inhibition decreases insulin-induced KGN cell proliferation and apoptosis by targeting PCGF1. Mol Med Rep 16:5706-5712 (2017). PubMed: 28849208
Images
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Western blot - Anti-PCGF1 antibody (ab84108)Anti-PCGF1 antibody (ab84108) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Additional bands at: 105 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
