Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families PAX

Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 24, 2021

Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7C2] to PAX9 - BSA and Azide free
  • Suitable for: WB, ICC
  • Reacts with: Mouse

You may also be interested in

Product image
Anti-PAX3 antibody (ab180754)
Product image
Anti-PAX3 antibody [OTI4D1] (ab236513)
Product image
Anti-PAX7 antibody [PAX7/497] - BSA and Azide free (ab212697)
Product image
Anti-PAX7 antibody [EPR3353] (ab92317)

Overview

  • Product name

    Anti-PAX9 antibody [7C2] - BSA and Azide free
    See all PAX9 primary antibodies
  • Description

    Rat monoclonal [7C2] to PAX9 - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Fusion protein corresponding to Mouse PAX9.

  • Positive control

    • WB: Mouse esophagus, Mouse skeletal muscle tissue. ICC: NIH/3T3 cells.
  • General notes

    ab255977 is the carrier-free version of ab28538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255977 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7C2
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • PAX
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Images

  • Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
    Western blot - Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
    All lanes : Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution

    Lane 1 : Mouse esophagus tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution (Goat Anti-Rat IgG (H+L), HRP)

    Predicted band size: 36 kDa



    This data was developed using ab28538, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 70 seconds

  • Immunocytochemistry - Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
    Immunocytochemistry - Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)

    This data was developed using ab28538 the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
    Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

  •  
  • Product image

    Anti-Peroxiredoxin 2/PRP antibody [EPR5154] (ab109367)

  •  
  • Product image

    Anti-PKN1 antibody [EPR18808] - BSA and Azide free (ab251202)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.