Anti-PAX9 antibody [7C2] - BSA and Azide free (ab255977)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7C2] to PAX9 - BSA and Azide free
- Suitable for: WB, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-PAX9 antibody [7C2] - BSA and Azide free
See all PAX9 primary antibodies -
Description
Rat monoclonal [7C2] to PAX9 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse PAX9.
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Positive control
- WB: Mouse esophagus, Mouse skeletal muscle tissue. ICC: NIH/3T3 cells.
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General notes
ab255977 is the carrier-free version of ab28538. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255977 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7C2 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-PAX9 antibody [7C2] (ab28538) at 1/1000 dilution
Lane 1 : Mouse esophagus tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution (Goat Anti-Rat IgG (H+L), HRP)
Predicted band size: 36 kDaThis data was developed using ab28538, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 70 seconds
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This data was developed using ab28538 the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling PAX9 with ab28538 at 1/20 (49.7 µg/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear staining in NIH/3T3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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