Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20598] to Parathyroid Hormone
Suitable for: IHC-P, IHC-Fr
Reacts with: Mouse, Rat

Product name

Anti-Parathyroid Hormone antibody [EPR20598]
See all Parathyroid Hormone primary antibodies
Description

Rabbit monoclonal [EPR20598] to Parathyroid Hormone
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC: Mouse parathyroid and kidney tissue; rat parathyroid and kidney tissue. IHC (Frozen): Mouse and rat parathyroid tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20598
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of rat parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) ab150077 at 1/1000 dilution.
Immunohistochemistry (Frozen sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution. Granular cytoplasmic staining on chief cells of mouse parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) ab150077 at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Cytoplasmic staining on distal convoluted tubules of rat kidney (PMID: 1846100) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of paraffin-embedded rat parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of rat parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Cytoplasmic staining on distal convoluted tubules of mouse kidney (PMID: 1846100) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

Immunohistochemical analysis of paraffin-embedded mouse parathyroid tissue labeling Parathyroid Hormone with ab213557 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051 ready to use). Granular cytoplasmic staining on chief cells of mouse parathyroid is observed, no staining on adjacent thyroid cells (PMID: 7615802). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Parathyroid Hormone antibody [EPR20598] (ab213557)

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