Anti-PAR1/Thrombin Receptor antibody (ab117749)
Key features and details
- Rabbit polyclonal to PAR1/Thrombin Receptor
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PAR1/Thrombin Receptor antibody
See all PAR1/Thrombin Receptor primary antibodies -
Description
Rabbit polyclonal to PAR1/Thrombin Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma. This antibody gave a positive signal in WB within Human Platelet tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P HumanWB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.Target
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Function
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development. -
Tissue specificity
Platelets and vascular endothelial cells. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated; probably mediating desensitization prior to the uncoupling and internalization of the receptor. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2149 Human
- Omim: 187930 Human
- SwissProt: P25116 Human
- Unigene: 482562 Human
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Alternative names
- CF2R antibody
- Coagulation factor II (thrombin) receptor antibody
- Coagulation factor II receptor antibody
see all
Images
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Western blot - Anti-PAR1/Thrombin Receptor antibody (ab117749)Anti-PAR1/Thrombin Receptor antibody (ab117749) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 116 kDa, 33 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesPAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab117749)IHC image of PAR1/Thrombin Receptor staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117749, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
References (0)
ab117749 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PAR1/Thrombin Receptor antibody (ab117749)Anti-PAR1/Thrombin Receptor antibody (ab117749) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 116 kDa, 33 kDa, 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesPAR1/Thrombin Receptor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAR1/Thrombin Receptor antibody (ab117749)
IHC image of PAR1/Thrombin Receptor staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117749, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
