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Cancer Oncoproteins/suppressors Oncoproteins Growth factor receptors

Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18413] to Pan Trk - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Pan Trk antibody [EPR18413] - BSA and Azide free
    See all Pan Trk primary antibodies
  • Description

    Rabbit monoclonal [EPR18413] to Pan Trk - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pan Trk aa 800 to the C-terminus (phospho Y820) (Cysteine residue). The exact sequence is proprietary. The original immunogen was phospho peptide but the antibody failed to specifically recognize the phospho site, recognizing phospho and nonphospho protein equally.
    Database link: Q16288-3

  • General notes

    Ab251000 is the carrier-free version of ab189903. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251000 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18413
  • Isotype

    IgG
  • Research areas

    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Cancer
    • Tumor biomarkers
    • Other

Images

  • Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa
    Observed band size: 140,30 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    TrkB is abundantly expressed in the central and peripheral nervous system. The 30KDa band is an intracellular fragment TrkB-ICD, and the 140KDa observed MW which is higher than the predicted one is due to the glycosylation modification.

  • Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    Western blot - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    All lanes : Anti-Pan Trk antibody [EPR18413] (ab189903) at 1/5000 dilution

    Lanes 1 & 3 & 5 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with empty vector (vector control)
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkA with His/Myc-tag
    Lane 4 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkB with His/Myc-tag
    Lane 6 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate transfected with TrkC with His/Myc-tag

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 94 kDa



    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 1 minute; Laned 3-6: 30 seconds.

  • Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    Immunoprecipitation - Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)

    This data was developed using ab189903, the same antibody clone in a different buffer formulation.

    TrkC (phospho Y820) was immunoprecipitated from 1 mg of mouse brain whole cell lysate with ab189903 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab189903 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10 µg (Input).

    Lane 2: ab189903 IP in mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab189903 in mouse brain whole cell lysate.

    Exposure time: 5 seconds.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)
    Anti-Pan Trk antibody [EPR18413] - BSA and Azide free (ab251000)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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