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Neuroscience Neurology process Growth and Development Neurotrophins

Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)

Price and availability

526 012 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [NORI146C] to p75 NGF Receptor
  • Suitable for: IHC-P, WB, IHC-Fr
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-p75 NGF Receptor antibody [NORI146C]
    See all p75 NGF Receptor primary antibodies
  • Description

    Rat monoclonal [NORI146C] to p75 NGF Receptor
  • Host species

    Rat
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Neuro-2a whole cell lysate. IHC-P: Mouse spleen and liver tissue. IHC-Fr: Mouse spleen tissue.
  • General notes

    ab271300 is the carrier-free version of ab271290. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab271300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    NORI146C
  • Isotype

    IgG2a
  • Research areas

    • Neuroscience
    • Neurology process
    • Growth and Development
    • Neurotrophins
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules

Images

  • Western blot - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Western blot - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Anti-p75 NGF Receptor antibody [NORI146C] (ab271290) at 0.755 µg/ml + Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 45 kDa


    Exposure time: 56 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271290).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271290).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD271 with ab271290 at 1/1000 dilution (0.755µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the peri-sinusoidal space in the mouse liver. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271290).

  • Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Immunohistochemistry (Frozen sections) - Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD271 with ab271290 at 1/100 dilution followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (7.55µg/ml) (Green). Positive staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271290).

  • Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)
    Anti-p75 NGF Receptor antibody [NORI146C] (ab271300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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