Anti-Otx1 antibody (ab25985)
Key features and details
- Rabbit polyclonal to Otx1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Otx1 antibody
See all Otx1 primary antibodies -
Description
Rabbit polyclonal to Otx1 -
Host species
Rabbit -
Specificity
We have not been able to test Rabbit polyclonal to Otx1 (ab25985) in Western Blot due to difficulties in accessing the appropriate lysates. We would appreciate any feedback on this antibody. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Chimpanzee, Gorilla, Catfish
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse Otx1.
Read Abcam's proprietary immunogen policy
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25985 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use at an assay dependent concentration. WB Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 37 kDa). This application has only been tested on recombinant protein.
Target
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Function
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. -
Tissue specificity
Expressed in brain. -
Sequence similarities
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Embryo. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5013 Human
- Entrez Gene: 18423 Mouse
- Entrez Gene: 25646 Rat
- Omim: 600036 Human
- SwissProt: P32242 Human
- SwissProt: Q53TG6 Human
- SwissProt: P80205 Mouse
- SwissProt: Q5SS54 Mouse
see all -
Alternative names
- FLJ38361 antibody
- Homeobox protein OTX 1 antibody
- Homeobox protein OTX1 antibody
see all
Images
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Western blot - Anti-Otx1 antibody (ab25985)Anti-Otx1 antibody (ab25985) at 1 µg/ml + OTX1 (Human) - Recombinant Protein at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25985 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Ab25985 recognizes the full length tagged recombinant protein which has an expected molecular weight of 75 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody (ab25985)This image is taken from an anonymous abreviewIHC-P image of Otx1 antibody (ab25985) on E11.5 mouse embryo sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using EDTA. These sections were then blocked in serum for 1 hour, before the Otx1 antibody was added at 1:100 dilution and left overnight at 4oC.
Datasheets and documents
References (4)
ab25985 has been referenced in 4 publications.
- Elwakeel E et al. Phenotypic Plasticity of Fibroblasts during Mammary Carcinoma Development. Int J Mol Sci 20:N/A (2019). PubMed: 31505876
- Xu PC et al. Myeloperoxidase influences the complement regulatory function of modified C-reactive protein. Innate Immun : (2013). PubMed: 24189124
- Saito T et al. Neocortical Layer Formation of Human Developing Brains and Lissencephalies: Consideration of Layer-Specific Marker Expression. Cereb Cortex : (2010). PubMed: 20624841
- Hadjivassiliou G et al. The application of cortical layer markers in the evaluation of cortical dysplasias in epilepsy. Acta Neuropathol : (2010). IHC-P ; Human . PubMed: 20411268
Images
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Western blot - Anti-Otx1 antibody (ab25985)Anti-Otx1 antibody (ab25985) at 1 µg/ml + OTX1 (Human) - Recombinant Protein at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab25985 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
Ab25985 recognizes the full length tagged recombinant protein which has an expected molecular weight of 75 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Otx1 antibody (ab25985) This image is taken from an anonymous abreviewIHC-P image of Otx1 antibody (ab25985) on E11.5 mouse embryo sections. The sections were fixed in Paraformaldehyde and underwent heat mediated antigen retrieval using EDTA. These sections were then blocked in serum for 1 hour, before the Otx1 antibody was added at 1:100 dilution and left overnight at 4oC.
