Anti-Osteoprotegerin antibody [bony-1] (ab18051)
![Anti-Osteoprotegerin antibody [bony-1] (ab18051)](https://www.abcam.com/ps/products/18/ab18051/Images/ab18051-26776-anti-osteoprotegerin-antibody-bony-1-flow-cytometry.jpg "Anti-Osteoprotegerin antibody [bony-1] (ab18051)")
Key features and details
- Rat monoclonal [bony-1] to Osteoprotegerin
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Osteoprotegerin antibody [bony-1]
See all Osteoprotegerin primary antibodies -
Description
Rat monoclonal [bony-1] to Osteoprotegerin -
Host species
Rat -
Specificity
Recognizes human OPG -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to amino acids 22-202 of Human Osteoprotegerin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from concentrated hybridoma tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
bony-1 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Anti-Osteoprotegerin antibody [bony-1] (ab18051)
FACS analysis of cells with ab18051.
HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min in 50µ l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide)
containing 1µ g/ml of ab18051. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by Flow Cytometry.
