Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Orexin A antibody [EPR22803-259] (ab255294)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22803-259] to Orexin A
Suitable for: Dot blot, IHC-P, IHC-Fr, ELISA
Reacts with: Mouse, Rat

Product name

Anti-Orexin A antibody [EPR22803-259]
See all Orexin A primary antibodies
Description

Rabbit monoclonal [EPR22803-259] to Orexin A
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Mouse
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Western blot: His-tagged mouse Orexin A recombinant protein (aa33-130). Dot blot: Orexin A peptide (aa33-65). IHC-P: Mouse and rat hypothalamus tissue. IHC-Fr: Mouse and rat hypothalamus tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22803-259
Isotype

IgG
Research areas

Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat hypothalamus tissue labeling Orexin A with ab255294 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on hypothalamic neurons (PMID: 9822755) is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Orexin A with ab255294 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat hypothalamus (PMID: 9419374) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab255294 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Dot Blot - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Dot blot analysis of Orexin A labeled with ab255294 at 1/1000 dilution.

Lane 1: Mouse Orexin A peptide (aa33-65).
Lane 2: Mouse Orexin B peptide (aa69-96).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
ELISA - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Direct ELISA antigen dose-response curve using ab255294 at 1 µg/mL. Antigen (mouse Orexin A peptide) concentration of 0.488-500 ng/mL. ab97048 Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) at 1/5000 was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse hypothalamus tissue labeling Orexin A with ab255294 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on hypothalamic neurons (PMID: 9822755) is observed. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Orexin A with ab255294 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hypothalamus (PMID: 9419374) is observed. Counterstained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab255294 for 15 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Anti-Orexin A antibody [EPR22803-259] (ab255294)

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