Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Orexin A antibody [EPR22803-259] (ab255294)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22803-259] to Orexin A
  • Suitable for: Dot blot, IHC-P, IHC-Fr, ELISA
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Orexin A antibody [EPR22803-259]
    See all Orexin A primary antibodies

  • Description

    Rabbit monoclonal [EPR22803-259] to Orexin A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Mouse
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Western blot: His-tagged mouse Orexin A recombinant protein (aa33-130). Dot blot: Orexin A peptide (aa33-65). IHC-P: Mouse and rat hypothalamus tissue. IHC-Fr: Mouse and rat hypothalamus tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat hypothalamus tissue labeling Orexin A with ab255294 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on hypothalamic neurons (PMID: 9822755) is observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Orexin A with ab255294 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in rat hypothalamus (PMID: 9419374) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab255294 for 15 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Dot Blot - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Dot Blot - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Dot blot analysis of Orexin A labeled with ab255294 at 1/1000 dilution.

    Lane 1: Mouse Orexin A peptide (aa33-65).
    Lane 2: Mouse Orexin B peptide (aa69-96).

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • ELISA - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    ELISA - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Direct ELISA antigen dose-response curve using ab255294 at 1 µg/mL. Antigen (mouse Orexin A peptide) concentration of 0.488-500 ng/mL. ab97048 Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) at 1/5000 was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Immunohistochemistry (Frozen sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse hypothalamus tissue labeling Orexin A with ab255294 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive staining on hypothalamic neurons (PMID: 9822755) is observed. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody [EPR22803-259] (ab255294)

    Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Orexin A with ab255294 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hypothalamus (PMID: 9419374) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab255294 for 15 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Anti-Orexin A antibody [EPR22803-259] (ab255294)
    Anti-Orexin A antibody [EPR22803-259] (ab255294)

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