Anti-OPRT antibody - C-terminal (ab186127)
Key features and details
- Rabbit polyclonal to OPRT - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-OPRT antibody - C-terminal
See all OPRT primary antibodies -
Description
Rabbit polyclonal to OPRT - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan
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Immunogen
Synthetic peptide within Human OPRT aa 400 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. (NP_000364.1).
Database link: P11172 -
Positive control
- HeLa, 293T, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates.
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General notes
This product was previously labelled as UMPS
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab186127 was affinity purified using an epitope specific to OPRT immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-OPRT antibody - C-terminal (ab186127)All lanes : Anti-OPRT antibody - C-terminal (ab186127) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-OPRT antibody - C-terminal (ab186127)Detection of OPRT in immunoprecipitates of 293T whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) using ab186127 at 6 µg per reaction for IP (Lane 1). For blotting, ab186127 was used at 1µg/ml. Lane 2: Control IgG IP.
Detection: Chemiluminescence with an exposure time of 3 minutes.
