Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-OPCML antibody [EPR22947-46] (ab238143)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22947-46] to OPCML
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-OPCML antibody [EPR22947-46]
See all OPCML primary antibodies
Description

Rabbit monoclonal [EPR22947-46] to OPCML
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human cerebellum, Human brain, Rat brain and Mouse brain lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat cerebral cortex tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22947-46
Isotype

IgG
Research areas

Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)

All lanes : Anti-OPCML antibody [EPR22947-46] (ab238143) at 1/1000 dilution

Lane 1 : Rat brain tissue lysate
Lane 2 : Mouse brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 38 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 3 minutes
Lane 2: 59 seconds

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)

All lanes : Anti-OPCML antibody [EPR22947-46] (ab238143) at 1/1000 dilution

Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human cerebellum treated with PNGase F tissue lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Human thymus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/500 dilution

Predicted band size: 38 kDa
Observed band size: 38,50 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Expsure times: 92 seconds.

This blot was developed using a higher sensitivity ECL substrate.

OPCML is glycosylated protein, the molecular weight observed is consistent with what has been described in the literature (PMID: 25538237).

Negative control: Human thymus (PMID: 12819783).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebral cortex (PMID: 10526147) is observed. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hippocampus. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebral cortex is observed. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-OPCML antibody [EPR22947-46] (ab238143)

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