Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-OPCML antibody [EPR22947-46] (ab238143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22947-46] to OPCML
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-OPCML antibody [EPR22947-46]
    See all OPCML primary antibodies

  • Description

    Rabbit monoclonal [EPR22947-46] to OPCML

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum, Human brain, Rat brain and Mouse brain lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat cerebral cortex tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)
    Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)
    All lanes : Anti-OPCML antibody [EPR22947-46] (ab238143) at 1/1000 dilution

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 38 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lane 1: 3 minutes
    Lane 2: 59 seconds

    This blot was developed using a higher sensitivity ECL substrate.

     

  • Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)
    Western blot - Anti-OPCML antibody [EPR22947-46] (ab238143)
    All lanes : Anti-OPCML antibody [EPR22947-46] (ab238143) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human cerebellum treated with PNGase F tissue lysate
    Lane 3 : Human brain tissue lysate
    Lane 4 : Human thymus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/500 dilution

    Predicted band size: 38 kDa
    Observed band size: 38,50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Expsure times: 92 seconds.

    This blot was developed using a higher sensitivity ECL substrate.

    OPCML is glycosylated protein, the molecular weight observed is consistent with what has been described in the literature (PMID: 25538237).

    Negative control: Human thymus (PMID: 12819783).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat cerebral cortex (PMID: 10526147) is observed. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse hippocampus. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control/ Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OPCML antibody [EPR22947-46] (ab238143)

    Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling OPCML with ab238143 at 1/1000 dilution (0.562 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cerebral cortex is observed. The section was incubated with ab238143 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-OPCML antibody [EPR22947-46] (ab238143)
    Anti-OPCML antibody [EPR22947-46] (ab238143)

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