Anti-OLA1 antibody (ab229090)
Key features and details
- Rabbit polyclonal to OLA1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-OLA1 antibody
See all OLA1 primary antibodies -
Description
Rabbit polyclonal to OLA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human OLA1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NTK5 -
Positive control
- IHC-P: Human colon carcinoma tissue. WB: HepG2 293T, A431, and HeLa whole cell lysates; Mouse brain lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-OLA1 antibody (ab229090)All lanes : Anti-OLA1 antibody (ab229090) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
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Western blot - Anti-OLA1 antibody (ab229090)Anti-OLA1 antibody (ab229090) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OLA1 antibody (ab229090)Paraffin-embedded human colon carcinoma tissue stained for OLA1 using ab229090 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-OLA1 antibody (ab229090)Anti-OLA1 antibody (ab229090) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 45 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-OLA1 antibody (ab229090)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OLA1 (green) using ab229090 at 1/200 dilution in ICC/IF. Cells were fixed in methanol.
Blue: Hoechst 33342 staining.
