Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
![Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)](https://www.abcam.com/ps/products/252/ab252384/Images/ab252384-2-202665Dot1.jpg "Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19847] to O-Linked N-Acetylglucosamine - BSA and Azide free
- Suitable for: WB, Dot blot, IP
- Reacts with: Human
Overview
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Product name
Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free
See all O-Linked N-Acetylglucosamine primary antibodies -
Description
Rabbit monoclonal [EPR19847] to O-Linked N-Acetylglucosamine - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa treated with DMSO (0.4%) as baseline control whole cell lysate; HeLa treated with 200 µM Ac45SGlcNAc for 24 hours whole cell lysate; HeLa treated with 200 µM Thiamet-G for 24 hours whole cell lysate; HEK-293t and HeLa whole cell lysate. IP: HeLa whole cell lysate.
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General notes
Ab252384 is the carrier-free version of ab202665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab252384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19847 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] (ab202665 1:1000 dilution).
Lane 1: O-linked N-acetylglucosamine (O-GlcNAc) peptide 10 ng.
Lane 2: Non-O-GlcNAc peptide 10 ng.
Blocking/Dilution buffer: 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 1:100,000 dilution).
Exposure time of 3 minutes.
The peptides were kindly provided by our collaborator Dr. Xing Chen, Peking University.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).
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![Immunoprecipitation - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)](https://www.abcam.com/ps/products/252/ab252384/Images/ab252384-1-202665IP1.jpg)
Immunoprecipitation - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)O-Linked N-Acetylglucosamine was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab202665 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202665 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input).
Lane 2: ab202665 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202665 in HeLa whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).
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![Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)](https://www.abcam.com/ps/products/252/ab252384/Images/ab252384-3-benefits-of-recombinant-antibodies.png)
Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
