Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19847] to O-Linked N-Acetylglucosamine - BSA and Azide free
  • Suitable for: WB, Dot blot, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free
    See all O-Linked N-Acetylglucosamine primary antibodies

  • Description

    Rabbit monoclonal [EPR19847] to O-Linked N-Acetylglucosamine - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Dot blot, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa treated with DMSO (0.4%) as baseline control whole cell lysate; HeLa treated with 200 µM Ac45SGlcNAc for 24 hours whole cell lysate; HeLa treated with 200 µM Thiamet-G for 24 hours whole cell lysate; HEK-293t and HeLa whole cell lysate. IP: HeLa whole cell lysate.

  • General notes

    Ab252384 is the carrier-free version of ab202665. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab252384 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19847

  • Isotype

    IgG

  • Research areas

Images

  • Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
    Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

    Dot Blot - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] (ab202665 1:1000 dilution).

    Lane 1: O-linked N-acetylglucosamine (O-GlcNAc) peptide 10 ng.

    Lane 2: Non-O-GlcNAc peptide 10 ng.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051 1:100,000 dilution).

    Exposure time of 3 minutes.

    The peptides were kindly provided by our collaborator Dr. Xing Chen, Peking University.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).

  • Immunoprecipitation - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
    Immunoprecipitation - Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

    O-Linked N-Acetylglucosamine was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with ab202665 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202665 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
    Lane 1: HeLa whole cell lysate 10 µg (Input).
    Lane 2: ab202665 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab202665 in HeLa whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab202665).

  • Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)
    Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-O-Linked N-Acetylglucosamine antibody [EPR19847] - BSA and Azide free (ab252384)

© 2021 Astana Biomed Group LLP. All rights reserved.