Anti-NUP205 antibody (ab157090)
Key features and details
- Rabbit polyclonal to NUP205
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NUP205 antibody -
Description
Rabbit polyclonal to NUP205 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region within amino acids 1750-1800 of Human NUP205 (NP_055950.1).
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Positive control
- 293T, HeLa and Jurkat whole cell lysate (ab7899)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157090 was affinity purified using an epitope specific to NUP205 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-NUP205 antibody (ab157090)All lanes : Anti-NUP205 antibody (ab157090) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 228 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-NUP205 antibody (ab157090)ab157090 at 1 µg/ml detecting NUP205 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes NUP205
Lane 2: ab157090 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.