Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)
![Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)](https://www.abcam.com/ps/products/278/ab278648/Images/ab278648-455811-AntiNucleophosmin-phospho-S4-antibody-NPMS4A1-flow-cytometry.JPG "Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [NPMS4-A1] to Nucleophosmin (phospho S4)
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1]
See all Nucleophosmin primary antibodies -
Description
Rabbit monoclonal [NPMS4-A1] to Nucleophosmin (phospho S4) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human Nucleophosmin (phospho S4). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P06748 -
Positive control
- WB: HeLa (treated with Nocodazole) cell extract. Flow cyt: K562 cells (treated with pervanadate).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
NPMS4-A1 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)
Flow cytometric analysis of K562 cells treated with imatinib (red) or treated with pervanadate (green) using ab278648 at 0.01 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotope Control for cells treated with imatinib (black) or treated with pervanadate (blue).
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![Western blot - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)](https://www.abcam.com/ps/products/278/ab278648/Images/ab278648-453883-ab2786483npms4a1helanocodazolevalidation.jpg)
Western blot - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)All lanes : Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648) at 0.1 µg/ml
Lane 1 : Human epithelial cell line from cervix adenocarcinoma (treated with nocodazole) cell extract
Lane 2 : Human epithelial cell line from cervix adenocarcinoma (untreated) cell extract
Predicted band size: 32 kDa
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![Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)](https://www.abcam.com/ps/products/278/ab278648/Images/ab278648-2-npms4a1peptideblocking.jpg)
Flow Cytometry - Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or treated with imatinib and blocked with phospho-peptide (black) or treated with pervanadate and blocked with phospho-peptide (gold) or treated with imatinib and blocked by non-phospho-peptide (dark blue) or treated with pervanadate and block with non-phospho-peptide (purple) using ab278648 at 0.01 ug/mL. -
![Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)](https://www.abcam.com/ps/products/278/ab278648/Images/ab278648-1-benefits-of-recombinant-antibodies.png)
Anti-Nucleophosmin (phospho S4) antibody [NPMS4-A1] (ab278648)