Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23808-72] to NRP2 - BSA and Azide free
  • Suitable for: ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free
    See all NRP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23808-72] to NRP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cyt, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG whole cell lysate. ICC/IF: U-87 MG cells. Flow Cyt: U-87 MG cells. IP: U-87 MG whole cell lysate.

  • General notes

    ab273591 is the carrier-free version of ab273584. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
    Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling NRP2 with ab273584 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in U-87 MG cell line. Negative control: MCF7 (PMID:18818766). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/202 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).

  • Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
    Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)

    Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / U-87 MG (Human glioblastoma-astrocytoma epithelial cell, Right) cells labelling NRP2 with ab273584 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: MCF7.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).

  • Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
    Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)

    NRP2 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate with ab273584 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273584 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab273584 IP in U-87 MG whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273584 in U-87 MG whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).

  • Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
    Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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