Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12716] to NPR-C - C-terminal
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NPR-C antibody [EPR12716] - C-terminal
    See all NPR-C primary antibodies

  • Description

    Rabbit monoclonal [EPR12716] to NPR-C - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    within Human NPR-C aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P17342

  • Positive control

    • Human fetal kidney, Human placenta, and HeLa lysates; HeLa cells.

  • General notes

     This product was previously labelled as Natriuretic Peptide Receptor C

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)

    All lanes : Human tissue lysate

    Lysates/proteins at 1/10 dilution per lane.

    Secondary
    All lanes : HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Flow Cytometry - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)

    Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

  • Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Western blot - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    All lanes : Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human placenta lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa

  • Flow Cytometry - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Flow Cytometry - Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)

    Flow Cytometric analysis of permeabilized HeLa cells labeling NPR-C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

  • Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)
    Anti-NPR-C antibody [EPR12716] - C-terminal (ab177954)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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