Anti-NOTCH3 antibody [4HCLC] (ab277799)
![Anti-NOTCH3 antibody [4HCLC] (ab277799)](https://www.abcam.com/ps/products/277/ab277799/Images/ab277799-1-antiNOTCH3recombinantpolyclonalantibody4hclcWB.jpg "Anti-NOTCH3 antibody [4HCLC] (ab277799)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [4HCLC] to NOTCH3
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-NOTCH3 antibody [4HCLC]
See all NOTCH3 primary antibodies -
Description
Rabbit recombinant multiclonal [4HCLC] to NOTCH3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NOTCH3 aa 2308-2321.
Database link: Q9UM47 -
Positive control
- WB: MCF7, U-87 MG, SH-SY5Y, NTERA-2, MDA-MB-231, T-47D, U-2 OS and A-431 whole cell extracts.
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General notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituent: 99.91% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
4HCLC -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NOTCH3 antibody (ab277799)All lanes : Anti-NOTCH3 antibody [4HCLC] (ab277799) at 2 µg/ml
Lane 1 : MCF7 whole cell extracts
Lane 2 : U-87 MG whole cell extracts
Lane 3 : SH-SY5Y whole cell extracts
Lane 4 : NTERA-2 whole cell extracts
Lane 5 : MDA-MB-23 whole cell extracts
Lane 6 : T-47D whole cell extracts
Lane 7 : U-2 OS whole cell extracts
Lane 8 : A-431 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution
Predicted band size: 244 kDa
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![Anti-NOTCH3 antibody [4HCLC] (ab277799)](https://www.abcam.com/ps/products/277/ab277799/Images/ab277799-2-benefits-of-recombinant-antibodies.jpg)
Anti-NOTCH3 antibody [4HCLC] (ab277799)