Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 09, 2021

Anti-NHP2L1 antibody [EPR11671] (ab181982)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11671] to NHP2L1
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
Reacts with: Human

Product name

Anti-NHP2L1 antibody [EPR11671]
See all NHP2L1 primary antibodies
Description

Rabbit monoclonal [EPR11671] to NHP2L1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Recombinant Human NHP2L1 protein (ab101947) can be used as a positive control in WB. HepG2, HeLa, Jurkat and MCF-7 cells. Human lung carcinoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11671
Isotype

IgG
Research areas

Western blot - Anti-NHP2L1 antibody [EPR11671] (ab181982)

All lanes : Anti-NHP2L1 antibody [EPR11671] (ab181982) at 1/20000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : MCF-7 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] (ab181982)

Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] (ab181982)

Immunofluorescence analysis of HeLa cells labeling NHP2L1 with ab181982 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor® 555) (1/200 dilution) and counter stained with Dapi.
Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (ab181982)

Immunoprecipitation analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/40 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
Flow Cytometry - Anti-NHP2L1 antibody [EPR11671] (ab181982)

Flow cytometry analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.
Anti-NHP2L1 antibody [EPR11671] (ab181982)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com