Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NHP2L1 antibody [EPR11671] (ab181982)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11671] to NHP2L1
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NHP2L1 antibody [EPR11671]
    See all NHP2L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11671] to NHP2L1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Recombinant Human NHP2L1 protein (ab101947) can be used as a positive control in WB. HepG2, HeLa, Jurkat and MCF-7 cells. Human lung carcinoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Western blot - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    All lanes : Anti-NHP2L1 antibody [EPR11671] (ab181982) at 1/20000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : MCF-7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 14 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NHP2L1 antibody [EPR11671] (ab181982)

    Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue labeling NHP2L1 with ab181982 at 1/1000 dilution followed by secondary Ready to use HRP Polymer for Rabbit IgG and hematoxylin counterstain.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Immunocytochemistry/ Immunofluorescence - Anti-NHP2L1 antibody [EPR11671] (ab181982)

    Immunofluorescence analysis of HeLa cells labeling NHP2L1 with ab181982 at 1/500 dilution followed by Goat anti rabbit IgG(Alexa Fluor® 555) (1/200 dilution) and counter stained with Dapi.

  • Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Immunoprecipitation - Anti-NHP2L1 antibody [EPR11671] (ab181982)

    Immunoprecipitation analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/40 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.

  • Flow Cytometry - Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Flow Cytometry - Anti-NHP2L1 antibody [EPR11671] (ab181982)

    Flow cytometry analysis of MCF-7 cells labeling NHP2L1 using ab181982 at 1/50 dilution. A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG.

  • Anti-NHP2L1 antibody [EPR11671] (ab181982)
    Anti-NHP2L1 antibody [EPR11671] (ab181982)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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