Anti-NFKBIL2 antibody (ab101898)
Key features and details
- Rabbit polyclonal to NFKBIL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NFKBIL2 antibody
See all NFKBIL2 primary antibodies -
Description
Rabbit polyclonal to NFKBIL2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human NFKBIL2 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab117409)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab101898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHorseCowDogLeaf frogApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 175 kDa (predicted molecular weight: 151 kDa).Target
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Function
Component of the MMS22L-TONSL complex, a complex that stimulates the recombination-dependent repair of stalled or collapsed replication forks. The MMS22L-TONSL complex is required to maintain genome integrity during DNA replication by promoting homologous recombination-mediated repair of replication fork-associated double-strand breaks. It may act by mediating the assembly of RAD51 filaments on ssDNA. Within the complex, may act as a scaffold. -
Tissue specificity
Expressed in heart, skeletal muscle and tracheal epithelial cells. -
Sequence similarities
Belongs to the Tonsoku family.
Contains 3 ANK repeats.
Contains 7 LRR (leucine-rich) repeats.
Contains 8 TPR repeats. -
Domain
The ANK repeats mediate the interaction with the MCM complex and histones, while the LRR repeats mediate the interaction with MMS22L. -
Cellular localization
Cytoplasm. Nucleus. Mainly nuclear. Localizes to DNA damage sites, accumulates at stressed replication forks. - Information by UniProt
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Database links
- Entrez Gene: 505102 Cow
- Entrez Gene: 475127 Dog
- Entrez Gene: 4796 Human
- Entrez Gene: 72749 Mouse
- Entrez Gene: 366953 Rat
- Omim: 604546 Human
- SwissProt: Q0P5G1 Cow
- SwissProt: Q96HA7 Human
see all -
Alternative names
- I kappa B related protein antibody
- I-kappa-B-related protein antibody
- IkappaBR antibody
see all
Images
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Western blot - Anti-NFKBIL2 antibody (ab101898)All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 151 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 67 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
Protocols
Datasheets and documents
References (2)
ab101898 has been referenced in 2 publications.
- Huang TH et al. The Histone Chaperones ASF1 and CAF-1 Promote MMS22L-TONSL-Mediated Rad51 Loading onto ssDNA during Homologous Recombination in Human Cells. Mol Cell 69:879-892.e5 (2018). PubMed: 29478807
- Saredi G et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature 534:714-718 (2016). WB . PubMed: 27338793
Images
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Western blot - Anti-NFKBIL2 antibody (ab101898)All lanes : Anti-NFKBIL2 antibody (ab101898) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 151 kDa
Observed band size: 175 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa, 67 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Ab101898 detects a band at approximately 170-kDa. While this differs to its predicted molecular weight of 151-kDa, this band has been observed in the literature (PMID:21055983).
