Anti-NeuN antibody - Neuronal Marker (ab128886)
Key features and details
- Rabbit polyclonal to NeuN - Neuronal Marker
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NeuN antibody - Neuronal Marker
See all NeuN primary antibodies -
Description
Rabbit polyclonal to NeuN - Neuronal Marker -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P RatWB MouseRatHuman
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse NeuN aa 300 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
(Peptide available asab155068) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on mouse cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
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Western blot - Anti-NeuN antibody - Neuronal Marker (ab128886)All lanes : Anti-NeuN antibody - Neuronal Marker (ab128886) at 5 µg/ml
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti Rabbit IR680 at 1/10000 dilution
Predicted band size: 34 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 48 kDa (possible isoform). We are unsure as to the identity of these extra bands.This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab128886 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on rat brain sections using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on dog cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on cat cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on marmoset (common) cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on goat cerebellum sections using ab128886 (1:2000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:2000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NeuN antibody - Neuronal Marker (ab128886) This image is courtesy of Carl Hobbs, King's College London, United KingdomIHC-P image of FOX3/NeuN staining on rat brain sections of Cortex (upper image) and lateral ventricle - SVZ (lower image) using ab128886 (1:3000). The sections were subjected to heat mediated antigen retrieval using citric acid pH6. The sections were blocked using 1% BSA for 10 mins using 21°C. The primary antibody was used at 1:3000 for 2 hours at 21°C. The secondary antibody used was goat polyclonal to rabbit IgG conjugated to biotin.
