Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nectin 3 antibody [EPR24274-4] - BSA and Azide free (ab278532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24274-4] to Nectin 3 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Nectin 3 antibody [EPR24274-4] - BSA and Azide free
    See all Nectin 3 primary antibodies

  • Description

    Rabbit monoclonal [EPR24274-4] to Nectin 3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: MDA-MB-231 whole cell lysate. WB: HCT 116 and MDA-MB-231 whole cell lysate.

  • General notes

    ab278532 is the carrier-free version of ab278526. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278532 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nectin 3 antibody [EPR24274A-4] - BSA and Azide free (ab278532)
    Western blot - Anti-Nectin 3 antibody [EPR24274A-4] - BSA and Azide free (ab278532)
    All lanes : Anti-Nectin 3 antibody [EPR24274-4] (ab278526) at 1/1000 dilution

    Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell)
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : U-937 (human histiocytic lymphoma monocyte) whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 61 kDa
    Observed band size: 83 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab278526, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The molecular weight  observed is consistent with what has been described in the literature (PMID:11024295, 26177744).

    Negative control: U-937 (PMID: 11024295).

    Exposure time: 158 seconds

     

  • Immunoprecipitation - Anti-Nectin 3 antibody [EPR24274A-4] - BSA and Azide free (ab278532)
    Immunoprecipitation - Anti-Nectin 3 antibody [EPR24274A-4] - BSA and Azide free (ab278532)

    This data was developed using ab278526, the same antibody clone in a different buffer formulation.

    Nectin 3 was immunoprecipitated from 0.35 mg MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab278526 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278526 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: MDA-MB-231 whole cell lysate 10 µg

    Lane 2: ab278526 IP in MDA-MB-231 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278526 in MDA-MB-231 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 119 seconds

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