Anti-NDUFA7 antibody (ab140871)
Key features and details
- Goat polyclonal to NDUFA7
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NDUFA7 antibody
See all NDUFA7 primary antibodies -
Description
Goat polyclonal to NDUFA7 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cow, Chimpanzee, Gorilla -
Immunogen
Synthetic peptide corresponding to Human NDUFA7 aa 39-62 (internal sequence) (Cysteine residue).
Sequence:PKLPVGPSHKLSNN
Database link: NP_004992.2 -
Positive control
- WB: Human, mouse, pig and rat heart lysates. IHC-P: Human heart tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue sections labelling NDUFA7 with ab140871 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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All lanes : Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lane 3 : Rat heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 13 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 13 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.