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Anti-NDUFA7 antibody (ab140871)

Anti-NDUFA7 antibody (ab140871)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to NDUFA7
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

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Overview

  • Product name

    Anti-NDUFA7 antibody
    See all NDUFA7 primary antibodies
  • Description

    Goat polyclonal to NDUFA7
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cow, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human NDUFA7 aa 39-62 (internal sequence) (Cysteine residue).
    Sequence:

    PKLPVGPSHKLSNN


    Database link: NP_004992.2
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human, mouse, pig and rat heart lysates. IHC-P: Human heart tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex I

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA7 antibody (ab140871)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NDUFA7 antibody (ab140871)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue sections labelling NDUFA7 with ab140871 at 5µg/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

  • Western blot - Anti-NDUFA7 antibody (ab140871)
    Western blot - Anti-NDUFA7 antibody (ab140871)
    All lanes : Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Mouse heart lysate (in RIPA buffer)
    Lane 3 : Rat heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Western blot - Anti-NDUFA7 antibody (ab140871)
    Western blot - Anti-NDUFA7 antibody (ab140871)
    Anti-NDUFA7 antibody (ab140871) at 0.1 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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