Anti-Nav1.5/SCN5A antibody (ab39992)
Key features and details
- Goat polyclonal to Nav1.5/SCN5A
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Nav1.5/SCN5A antibody
See all Nav1.5/SCN5A primary antibodies -
Description
Goat polyclonal to Nav1.5/SCN5A -
Host species
Goat -
Specificity
This antibody is expected to recognise both reported isoforms (NP_000326 and NP_932173). -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Synthetic peptide corresponding to Human Nav1.5/SCN5A aa 1548-1558 (internal sequence).
Sequence:ETDDQSPEKIN
-
Positive control
- IHC-P: Human cortex tissue; ICC/IF: U2OS cells.
-
General notes
This product was previously labelled as Nav1.5
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Nav1.5/SCN5A antibody (ab39992)
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells labelling Nav1.5/SCN5A with ab39992. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL), showing cytoplasmic/ER and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488® secondary antibody (2 µg/mL).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nav1.5/SCN5A antibody (ab39992)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain cortex tissue labelling Nav1.5/SCN5A with ab39992 at 10 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. Counterstained with hematoxylin.
Inset: Negative control showing staining of Human brain cortex with no primary antibody.
