Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11845] to NAP1L1 - BSA and Azide free
  • Suitable for: ICC, WB, IP, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free
    See all NAP1L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11845] to NAP1L1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250061 is the carrier-free version of ab178687 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250061 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Western blot - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 45 kDa



    This data was developed using ab178687, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    This data was developed using ab178687, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Immunocytochemistry - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    This data was developed using ab178687, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).
  • Flow Cytometry - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Flow Cytometry - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    This data was developed using ab178687, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Immunoprecipitation - Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    This data was developed using ab178687, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)
    Anti-NAP1L1 antibody [EPR11845] - BSA and Azide free (ab250061)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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