Anti-NAP1 (C12orf30)/p120 antibody (ab79490)
Key features and details
- Rabbit polyclonal to NAP1 (C12orf30)/p120
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAP1 (C12orf30)/p120 antibody -
Description
Rabbit polyclonal to NAP1 (C12orf30)/p120 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human NAP1 (C12orf30)/p120 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101294) -
Positive control
- This antibody gave a positive signal in HeLa and HepG2 whole cell lysates.
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General notes
Product previously known as NAP1 (C12orf30).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab79490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 112 kDa). ICC/IF Use a concentration of 10 µg/ml. Target
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Function
Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression. -
Sequence similarities
Belongs to the MDM20/NAA25 family.
Contains 4 TPR repeats. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 80018 Human
- Entrez Gene: 231713 Mouse
- Entrez Gene: 360811 Rat
- Omim: 612755 Human
- SwissProt: Q14CX7 Human
- SwissProt: Q8BWZ3 Mouse
- SwissProt: Q6QI44 Rat
- Unigene: 530941 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- C12orf30 antibody
- DKFZp667K2112 antibody
- FLJ1308 antibody
see all
Images
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Western blot - Anti-NAP1 (C12orf30)/p120 antibody (ab79490)All lanes : Anti-NAP1 (C12orf30)/p120 antibody (ab79490) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-NAP1 (C12orf30)/p120 antibody (ab79490)ICC/IF image of ab79490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79490, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 10µg/ml.
Protocols
Datasheets and documents
References (0)
ab79490 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-NAP1 (C12orf30)/p120 antibody (ab79490)All lanes : Anti-NAP1 (C12orf30)/p120 antibody (ab79490) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 112 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-NAP1 (C12orf30)/p120 antibody (ab79490)
ICC/IF image of ab79490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79490, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 10µg/ml.
