Anti-NAB1 antibody (ab241305)
Key features and details
- Rabbit polyclonal to NAB1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NAB1 antibody
See all NAB1 primary antibodies -
Description
Rabbit polyclonal to NAB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Syrian hamster
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Immunogen
Synthetic peptide within Human NAB1 aa 460-487. The exact sequence is proprietary. (NP_005957.2).
Database link: Q13506 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241305 was affinity purified using an epitope specific to NAB1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-NAB1 antibody (ab241305)
NAB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) usind ab241305 at 6 µg/mg lysate. Western bot was performed on the immunoprecipitate using ab241305 at 0.4 µg/ml.
Lane 1: ab241305 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 10 seconds.
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Western blot - Anti-NAB1 antibody (ab241305)All lanes : Anti-NAB1 antibody (ab241305) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 3 minutes
