Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Myosin antibody [A4.1025] (ab37484)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [A4.1025] to Myosin
Suitable for: IHC-P, IHC-Fr, ICC, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Myosin antibody [A4.1025]
See all Myosin primary antibodies
Description

Mouse monoclonal [A4.1025] to Myosin
Host species

Mouse

Tested Applications & Species

Application	Species
ICC	Mouse
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Full length native protein (purified). This information is considered to be commercially sensitive.
Positive control
- WB: Human, mouse and rat skeletal muscle tissue lysates; Differentiated C2C12 whole cell lysate IHC-P: Human skeletal and cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue. ICC: Differentiated C2C12 cells.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

A4.1025
Isotype

IgG2a
Research areas

Western blot - Anti-Myosin antibody [A4.1025] (ab37484)

Anti-Myosin antibody [A4.1025] (ab37484) at 0.1614 µg/ml + Human skeletal muscle tissue lysate at 20 µg

Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa
Observed band size: 223 kDa

Exposure time: 3 seconds

The extra bands under 223KDa were mainly caused by degradation (PMID: 3295257).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry - Anti-Myosin antibody [A4.1025] (ab37484)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Myosin (MYH1/2) with ab37484 at 3.228 µg/ml, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2µg/ml) (Green). Confocal image showing cytoplasmic staining in differentiated (treated) C2C12 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (2.5µg/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) at a 1/500 dilution (4µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (2µg/ml).
Western blot - Anti-Myosin antibody [A4.1025] (ab37484)

All lanes : Anti-Myosin antibody [A4.1025] (ab37484) at 0.807 µg/ml

Lane 1 : Mouse skeletal muscle tissue lysate
Lane 2 : Rat skeletal muscle tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa
Observed band size: 223 kDa

Exposure times: Lane 1: 3 secs; Lane 2: 1 sec.

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Myosin antibody [A4.1025] (ab37484)

All lanes : Anti-Myosin antibody [A4.1025] (ab37484) at 0.807 µg/ml

Lane 1 : C2C12 (mouse myoblasts) whole cell lysate
Lane 2 : Differentiated C2C12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 223 kDa
Observed band size: 223 kDa

Exposure time: 3 seconds

The expression profile observed is consistent with what has been described in the literature (PMID: 26010876).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 0.807 µg/ml, followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat skeletal muscle. The section was incubated with ab37484 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Myosin antibody [A4.1025] (ab37484)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle tissue labeling Myosin (MYH1/2) with ab37484 at 16.14 µg/ml followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Anti-Myosin antibody [A4.1025] (ab37484)

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