Anti-MYO1H antibody - C-terminal (ab219505)
Key features and details
- Goat polyclonal to MYO1H - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MYO1H antibody - C-terminal -
Description
Goat polyclonal to MYO1H - C-terminal -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse MYO1H aa 1000 to the C-terminus (C terminal) (Cysteine residue).
Database link: NP_001158045.1 -
Positive control
- NIH3T3 cell lysate; Human kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MYO1H antibody - C-terminal (ab219505)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling MYO1H with ab219505 at 5 μg/ml.
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Western blot - Anti-MYO1H antibody - C-terminal (ab219505)Anti-MYO1H antibody - C-terminal (ab219505) at 0.1 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 119 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.