Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)
Key features and details
- Rabbit polyclonal to Myelin oligodendrocyte glycoprotein
- Suitable for: WB, IHC-FoFr, IP
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Myelin oligodendrocyte glycoprotein antibody
See all Myelin oligodendrocyte glycoprotein primary antibodies -
Description
Rabbit polyclonal to Myelin oligodendrocyte glycoprotein -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-FoFr, IPmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Cow, Human, Common marmoset
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Immunogen
Synthetic peptide corresponding to Rat Myelin oligodendrocyte glycoprotein aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab32759)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab32760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). IHC-FoFr Use at an assay dependent concentration. IP Use a concentration of 5 µg/ml. Target
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Function
Mediates homophilic cell-cell adhesion (By similarity). Minor component of the myelin sheath. May be involved in completion and/or maintenance of the myelin sheath and in cell-cell communication. -
Tissue specificity
Found exclusively in the CNS, where it is localized on the surface of myelin and oligodendrocyte cytoplasmic membranes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100400400 Common marmoset
- Entrez Gene: 280863 Cow
- Entrez Gene: 4340 Human
- Entrez Gene: 17441 Mouse
- Entrez Gene: 24558 Rat
- Omim: 159465 Human
- SwissProt: Q29ZQ1 Common marmoset
- SwissProt: P55803 Cow
see all -
Alternative names
- BTN6 antibody
- BTNL11 antibody
- MGC26137 antibody
see all
Images
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Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) at 1 µg/ml
Lane 1 : Mouse Brain Whole Tissue Lysate
Lane 2 : Rat Brain Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)Image courtesy of Abreview from Sophie PezetAb32760 used on Mouse Cortex and Hippocampus in free floating sections using direct fluorescence. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat. -
Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)Myelin oligodendrocyte glycoprotein was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Myelin oligodendrocyte glycoprotein and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32760.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 27kDa: Myelin oligodendrocyte glycoprotein
Protocols
Datasheets and documents
References (15)
ab32760 has been referenced in 15 publications.
- Wang CY et al. BRCA1/BRCA2-containing complex subunit 3 controls oligodendrocyte differentiation by dynamically regulating lysine 63-linked ubiquitination. Glia 67:1775-1792 (2019). PubMed: 31184779
- Li H et al. Gene suppressing therapy for Pelizaeus-Merzbacher disease using artificial microRNA. JCI Insight 4:N/A (2019). PubMed: 31092737
- Yoshihara T et al. Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice. PLoS Genet 14:e1007545 (2018). PubMed: 30114188
- Wang CY et al. Function of B-Cell CLL/Lymphoma 11B in Glial Progenitor Proliferation and Oligodendrocyte Maturation. Front Mol Neurosci 11:4 (2018). WB ; Rat . PubMed: 29416501
- Beckmann N et al. Brain region-specific enhancement of remyelination and prevention of demyelination by the CSF1R kinase inhibitor BLZ945. Acta Neuropathol Commun 6:9 (2018). PubMed: 29448957
Images
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Western blot - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)All lanes : Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) at 1 µg/ml
Lane 1 : Mouse Brain Whole Tissue Lysate
Lane 2 : Rat Brain Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 27 kDa
Observed band size: 27 kDa
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760) Image courtesy of Abreview from Sophie PezetAb32760 used on Mouse Cortex and Hippocampus in free floating sections using direct fluorescence. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
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Immunoprecipitation - Anti-Myelin oligodendrocyte glycoprotein antibody (ab32760)Myelin oligodendrocyte glycoprotein was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to Myelin oligodendrocyte glycoprotein and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab32760.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 27kDa: Myelin oligodendrocyte glycoprotein
