Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23548-120] to mtTFA - BSA and Azide free
  • Suitable for: IP, ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free
    See all mtTFA primary antibodies

  • Description

    Rabbit monoclonal [EPR23548-120] to mtTFA - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C2C12, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse cerebellum, heart and kidney tissue lysates;, Rat cerebellum and heart tissue lysates. ICC/IF: C2C12 and PC-12 cells. Flow Cyt: C2C12 cells.

  • General notes

    ab273508 is the carrier-free version of ab252432. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Flow Cytometry - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Flow Cytometry - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labelling mtTFA with ab252432 at 1/600 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252432).

  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252432).

  • Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

    mtTFA was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.

    Lane 1: C2C12 whole cell lysate (Input)
    Lane 2: ab252432 IP in C2C12 cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in C2C12 whole cell lysate

    Blocking /Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 32 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252432).

  • Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Immunoprecipitation - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

    mtTFA was immunoprecipitated from 0.35 mg PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate using ab252432 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab252432 at 1/500 dilution, followed by VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution.

    Lane 1: PC-12 whole cell lysate (Input)
    Lane 2: ab252432 IP in PC-12 cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab252432 in PC-12 whole cell lysate

    Blocking /Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 32 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252432).

  • Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling mtTFA with ab252432 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252432).

  • Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)
    Anti-mtTFA antibody [EPR23548-120] - BSA and Azide free (ab273508)

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