Anti-mtTFA antibody (ab272885)
Key features and details
- Rabbit polyclonal to mtTFA
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-mtTFA antibody
See all mtTFA primary antibodies -
Description
Rabbit polyclonal to mtTFA -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human mtTFA.
Database link: Q00059 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, PC-12 and Rat2 whole cell lysate. IHC-P: Human breast cancer tissue. ICC/IF: HeLa and NTERA-2 cl.D1 [NT2/D1] cells. IP: HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mtTFA antibody (ab272885)Paraffin-embedded human breast cancer tissue stained for mtTFA using ab272885 at 1/250 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab272885)4% PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.
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Immunoprecipitation - Anti-mtTFA antibody (ab272885)Immunoprecipitation - Anti-mtTFA antibody (ab272885).
Sample: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
A. 40 μg HEK-293T whole cell lysate.
B. Control with 2 μg of preimmune rabbit IgG
C. Immunoprecipitation of mtTFA protein by 2 μg of ab272885
12% SDS-PAGE
The immunoprecipitated mtTFA protein was detected by ab272885 diluted at 1:1000, followed by anti-rabbit IgG-HRP secondary antibody.
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Western blot - Anti-mtTFA antibody (ab272885)Anti-mtTFA antibody (ab272885) at 1/1000 dilution + Neuro-2a (mouse neuroblastoma cell line) whole cell lysate at 30 µg12% SDS-PAGE
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/500 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-mtTFA antibody (ab272885)2% paraformaldehyde/culture medium fixed NTERA-2 cl.D1 [NT2/D1] (Human malignant pluripotent embryonic carcinoma cell line) cells stained for mtTFA using ab272885 at 1/500 dilution in ICC/IF. Blue: Hoechst 33342 staining.
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Western blot - Anti-mtTFA antibody (ab272885)All lanes : Anti-mtTFA antibody (ab272885) at 1/3000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : mTFA shRNA transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG12% SDS-PAGE
