Anti-MMP9 antibody [56-2A4] (ab58803)
![Anti-MMP9 antibody [56-2A4] (ab58803)](https://www.abcam.com/ps/products/58/ab58803/Images/ab58803-218393-ab58803.jpg "Anti-MMP9 antibody [56-2A4] (ab58803)")
Key features and details
- Mouse monoclonal [56-2A4] to MMP9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-MMP9 antibody [56-2A4]
See all MMP9 primary antibodies -
Description
Mouse monoclonal [56-2A4] to MMP9 -
Host species
Mouse -
Specificity
Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13. The antibody recognizes precursor (92 kDa) and active (83 kDa) forms of human and guinea pig MMP-9. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human MMP9 aa 626-644.
Sequence:LFSGRRLWRFDVKAQMVDP
Database link: P14780 -
Positive control
- Breast carcinoma tissue. Conditioned medium of human fibrosarcoma HT1080 cells The protein is secreted so you may need to concentrate the protein from cell culture media. Concentrate protein by TCA, acetone or ammonium sulphate precipitation. HL60 cell lysate, U937 cell lysate, HT1080 cell lysate, human spleen lysate
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.1% Sodium azide
Constituents: 1.64% Sodium phosphate, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Is purified. -
Clonality
Monoclonal -
Clone number
56-2A4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-MMP9 antibody [56-2A4] (ab58803)
1:500 dilution of ab58803 detected MMP-9 on 10 µg of Human spleen lysates. Arrow indicates protein MMP-9 (~92 kDa). Lower bands indicate active forms of MMP-9
