Anti-MLC1 antibody (ab130770)
Key features and details
- Rabbit polyclonal to MLC1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-MLC1 antibody
See all MLC1 primary antibodies -
Description
Rabbit polyclonal to MLC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
corresponding to MLC1.
(Peptide available asab169568) -
Positive control
- This antibody gave a positive signal in the following Mouse Tissue lysates: Brain; Forebrain; Cortex; Hippocampus; Cerebellum; Olfactory Bulb.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatChinese hamsterApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 42 kDa).Target
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Function
Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx. -
Tissue specificity
Expressed in the brain, with highest levels found in the amygdala, nucleus caudatus, thalamus and hippocampus. -
Involvement in disease
Leukoencephalopathy, megalencephalic, with subcortical cysts, 1 -
Cellular localization
Membrane. Cell membrane. Cytoplasm, perinuclear region. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 170790 Mouse
- Entrez Gene: 315215 Rat
- SwissProt: Q8VHK5 Mouse
- Unigene: 32780 Mouse
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Alternative names
- KIAA0027 antibody
- LVM antibody
- Megalencephalic leukoencephalopathy with subcortical cysts 1 antibody
see all
Images
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Western blot - Anti-MLC1 antibody (ab130770)All lanes : Anti-MLC1 antibody (ab130770) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Mouse Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 240 kDa, 27 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab130770 detects a band at 44 + 77 kDa, while this differs to its predicted molecular weight of 42 kDa, the banding pattern observed is consistent with what has been described in the literature PMID: 15367490. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130770 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (1)
ab130770 has been referenced in 1 publication.
- Hwang J et al. Plasma membrane localization of MLC1 regulates cellular morphology and motility. Mol Brain 12:116 (2019). PubMed: 31888684
Images
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Western blot - Anti-MLC1 antibody (ab130770)All lanes : Anti-MLC1 antibody (ab130770) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Mouse Olfactory Bulb Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
Additional bands at: 240 kDa, 27 kDa, 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab130770 detects a band at 44 + 77 kDa, while this differs to its predicted molecular weight of 42 kDa, the banding pattern observed is consistent with what has been described in the literature PMID: 15367490. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130770 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
