Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)
![Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)](https://www.abcam.com/ps/products/278/ab278704/Images/ab278704-2-20170517mkk7r4f9img.jpg "Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MKK7S271T275-R4F9] to MKK7 (phospho S271 + T275)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9]
See all MKK7 primary antibodies -
Description
Rabbit monoclonal [MKK7S271T275-R4F9] to MKK7 (phospho S271 + T275) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human MKK7 (phospho S271 + T275). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14733 -
Positive control
- WB: THP-1 (treated with sorbitol) cell extract. Flow cyt: C6 cells (treated with EGF); C2C12 cells (treated with staurosporine).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MKK7S271T275-R4F9 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)
Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with EGF (green) using ab278704 at 0.5 µg/mL.
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![Western blot - Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)](https://www.abcam.com/ps/products/278/ab278704/Images/ab278704-455170-anti-mkk7-phospho-s271-t275-antibody-mkkK7s271t275-r4f9-western-blot-sorbitol-treated-thp1-human.jpg)
Western blot - Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)All lanes : Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704) at 0.1 µg/ml
Lane 1 : THP-1 (human monocytic leukemia cell line) treated with sorbitol, whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) untreated, whole cell lysate
Predicted band size: 47 kDa
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![Flow Cytometry - Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)](https://www.abcam.com/ps/products/278/ab278704/Images/ab278704-4-mkk7r4f9c2c12.jpg)
Flow Cytometry - Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 1 µg/mL of isotype control (orange) or untreated (red) or treated with staurosporine (green) using ab278704 at 1 µg/mL.
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![Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)](https://www.abcam.com/ps/products/278/ab278704/Images/ab278704-1-benefits-of-recombinant-antibodies.png)
Anti-MKK7 (phospho S271 + T275) antibody [MKK7S271T275-R4F9] (ab278704)