Anti-MHC class I antibody [R1-21.2] (ab281904)
![Anti-MHC class I antibody [R1-21.2] (ab281904)](https://www.abcam.com/ps/products/281/ab281904/Images/ab281904-2-AntiMHC-class-I-antibody-westernblot-splenocytesmouse.jpg "Anti-MHC class I antibody [R1-21.2] (ab281904)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [R1-21.2] to MHC class I
- Suitable for: WB, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-MHC class I antibody [R1-21.2]
See all MHC class I primary antibodies -
Description
Rat monoclonal [R1-21.2] to MHC class I -
Host species
Rat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC class I. Raised by immunizing Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunized rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
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Positive control
- WB: Mouse thymus tissue lysate. ICC: Mouse splenocytes.
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General notes
ab281904 is the recombinant rat verssion of ab281902.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R1-21.2 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-MHC class I antibody [R1-21.2] (ab281904)
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![Immunocytochemistry - Anti-MHC class I antibody [R1-21.2] (ab281904)](https://www.abcam.com/ps/products/281/ab281904/Images/ab281904-1-AntiMHC-class-I-antibody-imunocytochemistry-splenocytesmouse.jpg)
Immunocytochemistry - Anti-MHC class I antibody [R1-21.2] (ab281904)This data was developed using the rabbit chimeric version (ab281902).
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes labelling MHC-I with ab281902 at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/ top-left). Image showing membrane staining in a subset of cells. The nuclear counter stain is DAPI (blue). The isotype control was anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody (bottom-right). Mouse splenocytes were immobilized on Shi-fix™ cover-slips.