Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MGST1 antibody [EPR7934] - BSA and Azide free
    See all MGST1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7934] to MGST1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver tissue.

  • General notes

    ab232469 is the carrier-free version of ab131059 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Microsomal Glutathione S-transferase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling MGST1 with ab131059 antibody at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Flow Cytometry - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling MGST1 with purified ab131059 at 1:40 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunocytochemistry/ Immunofluorescence - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Microsomal Glutathione S-transferase 2 with Purified ab131059 at 1:100 dilution. Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 µg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    OI-RD Scanning - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling MGST1 with Purified ab131059 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131059).

  • Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)
    Anti-MGST1 antibody [EPR7934] - BSA and Azide free (ab232469)

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