Anti-MEF2D antibody (ab246884)
Key features and details
- Rabbit polyclonal to MEF2D
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MEF2D antibody
See all MEF2D primary antibodies -
Description
Rabbit polyclonal to MEF2D -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MEF2D aa 350 to the C-terminus.
Database link: Q14814 -
Positive control
- IHC-P: Human skeletal muscle, kidney and placenta tissues. WB: THP-1, NIH/3T3 and NBT-II cell lysates. ICC/IF: U-2 OS cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MEF2D antibody (ab246884)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for MEF2D (green) using ab246884 at 4 µg/ml in ICC/IF.
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Western blot - Anti-MEF2D antibody (ab246884)All lanes : Anti-MEF2D antibody (ab246884) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246884)Formalin-fixed, paraffin-embedded human kidney tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246884)Formalin-fixed, paraffin-embedded human placenta tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246884)Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for MEF2D with ab246884 at a 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2D antibody (ab246884)Formalin-fixed, paraffin-embedded human skeletal liver shows absence of immunoreactivity for MEF2D when incubated with ab246884 at a 1/50 dilution (negative control).
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Western blot - Anti-MEF2D antibody (ab246884)Anti-MEF2D antibody (ab246884) at 0.4 µg/ml + THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 56 kDa
Western blot analysis in human cell line THP-1.