Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)
![Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)](https://www.abcam.com/ps/products/202/ab202866/Images/ab202866-352435-ab202866AP48211485pcMilk05concPublish.jpg "Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)")
Key features and details
- Mouse monoclonal [Ab3] to MCPyV_gp3 large T antigen
- Suitable for: WB, ICC/IF
- Isotype: IgG1
Overview
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Product name
Anti-MCPyV_gp3 large T antigen antibody [Ab3]
See all MCPyV_gp3 large T antigen primary antibodies -
Description
Mouse monoclonal [Ab3] to MCPyV_gp3 large T antigen -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Immunogen
Recombinant fragment (GST-tag) within MCPyV_gp3 large T antigen aa 1-300 (N terminal). The exact sequence is proprietary.
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Epitope
The epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601). -
Positive control
- WB: MKL-1 whole cell lysate. ICC: MKL-1 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Ab3 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Western blot - Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)All lanes : Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866) at 0.5 µg/ml
Lane 1 : MKL-1 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HEK293 whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 48 kDa why is the actual band size different from the predicted?This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)](https://www.abcam.com/ps/products/202/ab202866/Images/ab202866-381163-antimcpyvgp3largetantigenab3iccmkl1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MCPyV_gp3 large T antigen antibody [Ab3] (ab202866)ab202866 staining MCPyV_gp3 large T antigen in MKL-1 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202866 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).