Anti-MCP3 antibody [18] - BSA and Azide free (ab277128)
![Anti-MCP3 antibody [18] - BSA and Azide free (ab277128)](https://www.abcam.com/ps/products/277/ab277128/Images/ab277128-2-AntiMCP3-antibody-Flow-cyt-THP1-cells.jpg "Anti-MCP3 antibody [18] - BSA and Azide free (ab277128)")
Key features and details
- Mouse monoclonal [18] to MCP3 - BSA and Azide free
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-MCP3 antibody [18] - BSA and Azide free
See all MCP3 primary antibodies -
Description
Mouse monoclonal [18] to MCP3 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC Human
-
Immunogen
Recombinant full length protein corresponding to Human MCP3 aa 24-99. Full-length mature chain. Genbank: NP_006264.2
Database link: P80098 -
Positive control
- Flow cyt: THP-1 cells. ICC: SKBR3 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
18 -
Isotype
IgG1 -
Research areas
Images
-
Flow Cytometry - Anti-MCP3 antibody (ab277128)
Flow cytometric analysis of CCL7 expression on THP-1 (human monocytic leukemia cell line) cells. The cells were stained with ab277128 at 1/25 dilution, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
-
Immunocytochemistry - Anti-MCP3 antibody (ab277128)Immunofluorescence staining of Human CCL7 in SKBR3 (human mammary gland adenocarcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277128 at 1/60 dilution. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), counterstained with DAPI (blue).