Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCH antibody [EPR23778-94] (ab274415)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23778-94] to MCH
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MCH antibody [EPR23778-94]
    See all MCH primary antibodies

  • Description

    Rabbit monoclonal [EPR23778-94] to MCH

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes the PMCH chain.

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human hypothalamus tissue; Mouse hypothalamus tissue; Rat hypothalamus tissue. IHC-Fr: Mouse hypothalamus tissue; Rat hypothalamus tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23778-94

  • Isotype

    IgG

Images

  • Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
    Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)

    Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
    Immunohistochemistry (Frozen sections) - Anti-MCH antibody [EPR23778-94] (ab274415)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/500 (1.084 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse hypothalamus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)

    Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCH antibody [EPR23778-94] (ab274415)

    Immunohistochemical analysis of paraffin-embedded Human hypothalamus tissue labeling PMCH with ab274415 at 1/2000 (0.271 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human hypothalamus (PMID: 30420052). The section was incubated with ab274415 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-MCH antibody [EPR23778-94] (ab274415)
    Anti-MCH antibody [EPR23778-94] (ab274415)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.